Intended use of the test kit
This One-Step Test is intended to use as a practical/routine screening test, that can be done in a few minutes. This test kit is designed to detect Leishmania antibody by use of a Rapid Immunochromatic Assay.
Principle of the test kit
This Leishmania One-Step Test is based on a chromatographic test strip and the detection of antibodies against Leishmania. A specific monoclonal anti-dog immunoglobulin is conjugated to colloidal gold particles and purified Leishmania proteins are immobilized on the strip in the test zone “T”. Leishmania antibodies in a sample that is applied to the strip at the sample zone “S” will bind to the gold particles which then migrate to zone “T”. A colour change in zone “T” indicates a positive test. Leishmania protein is also immobilized on the strip in the control zone “C”, which binds the gold conjugate to indicate that the test is working properly.