Intended use of the test kit
The Leishmania ELISA test kit is designed to detect antibodies against Leishmania proteins. Leishmania proteins are attached to the solid phase. After washing the strips are incubated with the samples to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound dog antibodies to Leishmania proteins. After incubation and rinsing the substrate is added and the optical density is measured at 450 nm.
Principle of the test kit
The test is based on the reaction of Leishmania proteins with dog antibodies. To this end Leishmania proteins have been coated to a 96-well microtiter plate.
The diluted dog serum/plasma sample is added to the wells of the coated plate.
The sample is added (diluted 1:100) to the wells of the coated plate.
The sample also can be titrated using a 3-step dilution, starting with a dilution 1:100 (> 1:300 > 1:900 > 1:2700).
After washing the bound dog antibodies are detected by a HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of Leishmania antibodies in the serum/plasma sample.