Leishmania Antibody SRE

 167,36

An SRE based on a partially purified antigen to detect antibodies against Leishmania in serum samples or plasma samples.

SKU: D3214-AB01 Categories: ,

Description

Intended use of the test kit

The Leishmania SRE test kit is designed to detect antibodies against Leishmania proteins. Leishmania proteins are attached to the solid phase. After washing, the strips are incubated with the samples to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound dog antibodies to Leishmania proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of Leishmania proteins with dog antibodies. To this end Leishmania proteins have been coated to a 32-well microtiter plate. The diluted dog serum/plasma sample is added to the wells of the coated plate. After washing, the bound dog antibodies are detected by a HRPO conjugated anti-species conjugate. The color reaction in the wells is directly related to the concentration of Leishmania antibodies in the serum/plasma sample.

Protocols

Leishmania Antibody SRE

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