Intended use of the test kit
The feline T4 total ELISA is designed to detect T4 total in individual serum and plasma samples. For this purpose monoclonal anti-T4 total antibodies attached to the plate will catch the thyroxin in the sample to be tested. The thyroxin present in the sample will compete with the specific biotin binding conjugate. After incubation the ELISA will be washed to remove unbound thyroxin. Peroxidase marked streptavidine conjugate will be added to the ELISA wells. After incubation the ELISA will be washed to remove unbound streptavidine. Substrate will be added to the ELISA wells and the colour development is inversely directly related with the quantity of bound thyroxin.
Principle of the test kit
The test is bases on the competition of thyroxin in the sample to be tested, with known biotin marked thyroxin conjugate. To this end monoclonal anti-T4 total antibodies are coated to a 96 well microtiter strip plate.
The feline serum/plasma sample is added together with the biotin marked T4 to the wells of the coated plate.
Colour reaction in the wells is inversely directly related to the concentration of thyroxin in the serum/plasma sample.