Intended use of the test kit
The Chlamydia SRE kit is designed to detect antibodies against Chlamydia antigens. To this end Chlamydia proteins are attached to the solid phase. After washing, the plates are incubated with the avian sera to be tested. The plates are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound avian antibodies to Chlamydia antigen. After incubation and rinsing, the substrate is added and the optical density is measured at 620 nm.
Principle of the test kit
The test is based on the reaction of Chlamydia proteins with polyclonal antibodies. To this end, Chlamydia proteins have been coated to a 32-well microtiter plate.
After washing, the bound bird antibodies are detected by a HRPO conjugated anti-species conjugate.
The colour reaction in the wells is directly related to the concentration of Chlamydia antibodies in the serum/plasma sample.