Intended use of the test kit
The anti-thyroglobulin kit is develop to detect antibodies against thyroglobulin and thyroglobulin complexes. To this end thyroglobulin and thyroglobulin complexes are bound to a solid phase.
After washing, the plates are incubated with the samples to be tested. The plates are washed after incubation to remove unbound material.
A HRPO conjugate is added to detect bound antibodies. After incubation and washing, the substrate is added and the optical density is measured at 620 nm.
Principle of the test kit
The test is based on the reaction of polyclonal antibodies reactive against thyroglobulin and thyroglobulin complexes.
The colour reaction in the wells is directly related to the concentration of antibodies in the serum/plasma sample.