Intended use of the test kit
This One-Step Test is intended to use as practical/routine screening test that can be done in a few minutes. This test kit is designed to detect African swine fever virus antigen by use of a Rapid Immunochromatic Assay.
Principle of the test kit
The ASFV antibody One-Step is based on a chromatographic principle in which a monoclonal antibody reacts with epitopes of the ASFV virus antibody. A monoclonal antibody is conjugated to colloidal gold particles and a monoclonal antibody is immobilized on the test strip in the test zone “T”. ASF antigen in the sample that is applied to the test strip at the sample zone “S”, will bind to the colloidal gold particles which then migrate to zone” T”. A colour change in zone “T” indicates a positive test. Colloidal gold particles are also immobilized on the test strip in the control zone “C”, to indicate that the test is working properly.