Canine T4 Total ELISA

Description

Intended use of the test kit

The Canine T4 total ELISA is designed to detect total T4 in individual serum and plasma samples. For this purpose monoclonal anti-T4 total antibodies attached to the plate will catch the bound and unbound thyroxin (T4) in the sample to be tested. The thyroxin present in the sample will compete with the specific biotin-marked thyroxin conjugate. After incubation the ELISA will be washed to remove unbound thyroxin. Peroxidase marked streptavidine conjugate will be added to the ELISA wells. After incubation the ELISA will be washed to remove unbound streptavidine. Substrate will be added to the ELISA wells and the color development is inversely related with the quantity of bound thyroxin.

Principle of the test kit

The test is based on the competition of thyroxin in the sample to be tested, with known biotin marked thyroxin conjugate. To this end monoclonal anti-T4 total antibodies are coated to a 96-well microtiter strip plate.
The canine serum/plasma sample is added together with the biotin marked T4 to the wells of the coated plate.
Color reaction in the wells is inversely related to the concentration of thyroxin in the serum/plasma sample.

Protocol

Canine T4 total