Intended use of the test kit
The canine total T4 SRE is designed to detect total T4 in individual serum and plasma samples. For this purpose monoclonal anti-T4 antibodies attached to the plate will catch the thyroxin (T4) in the sample to be tested. The thyroxin present in the sample will compete with the specific biotin-marked thyroxin conjugate. After incubation the plate will be washed to remove unbound thyroxin. Peroxidase marked streptavidine conjugate will be added to the SRE wells. After incubation the SRE plate will be washed to remove unbound streptavidine. Substrate will be added to the SRE wells and the colour development is inversely correlated with the quantity of T4 (thyroxine) in the sample.
Principle of the test kit
The test is based on the competition of thyroxin in the sample to be tested, with known biotin marked thyroxin conjugate. To this end monoclonal anti-T4 total antibodies are coated to a 32-well microtiter strip plate.
The canine serum/plasma is added together with the biotin marked T4 to the wells of the coated plate.
Colour reaction in the wells is inversely related to the concentration of thyroxin in the serum/plasma sample.