Feline Leukemia Virus gp70 IgG antibody ELISA

Description

Intended use of the test kit

The peptide based immunoassay (ELISA) test kit is designed to detect gp70 antibodies in serum samples. The kit procedure is based on a solid phase ELISA. To this end a peptide corresponding to a neutralizing immuno dominant epitope of gp70 is attached to the solid phase. After washing, a diluted sample is added. After incubation and before the addition of anti-species conjugate, unbound materials are removed by rinsing. After incubation and rinsing, the substrate is added and the optical density is measured at 450nm.

Principle of the test kit

The test is based on the reaction of FeLV-gp70 antibodies present in the test sample with immobilized peptide antigen. To this end, gp70 peptides have been coated to the wells of a 96 well microtiter-strip-plate.

• Qualitative
  The sample is added (diluted 1:150) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (> 1:90 > 1:270 > 1:810).

After washing, samples are added to the wells and will bind to the gp70 peptide molecules which have been attached to the solid phase. The bound antibody is detected by a horseradish peroxidase (HRPO) conjugated anti-species conjugate. Color reaction in the wells is directly related to the concentration of FeLV-gp70 antibodies in the sample.

Protocol

Feline Leukemia Virus gp70 antibody ELISA