Intended use of the test kit
The FeLV-gp70 antibody ELISA is designed to detect gp70 antibodies in serum and plasma samples. The kit procedure is based on a solid phase ELISA. When a standard gp70 antigen suspension is added, the gp70 molecule is bound by monoclonal antibodies attached to the solid phase. Unbound materials are removed by rinsing. A diluted serum/plasma sample is then added. After incubation and before the addition of peroxidase labelled anti-species conjugate, unbound materials are removed by rinsing. After incubation and rinsing, the substrate is then added and the optical density is measured at 450 nm.
Principle of the test kit
The test is based on the reaction of FeLV-gp70 antibodies present in the test sample with immobilized FeLV gp70 antigen. To this end, monoclonal anti-gp70 antibodies have been coated to the wells of a 96 well microtiter plate.
The FeLV-gp70 antigen suspension is added to the wells and is captured by the coated monoclonal antibodies.
The sample is added (diluted 1:150) to the wells of the coated plate.
The sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (> 1:150 > 1:450 > 1:1350).
After washing, samples are added to the wells and will bind to the gp70 molecules, which have been caught. The bound antibody is detected by a horseradish peroxidase (HRPO) conjugated anti-species conjugate.
Color reaction in the wells is directly related to the concentration of FeLV-gp70 antibodies in the sample.