Helicobacter IgG antibody ELISA

Description

Intended use of the test kit

The Helicobacter ELISA test kit is designed to detect antigens against Helicobacter antigens. Helicobacter antigens are attached to the solid phase. After washing, the strips are incubated with the samples to be tested. The strips are washed, after incubation, to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound dog antibodies to Helicobacter antigens. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of Helicobacter antigens with polyclonal dog antibodies. To this end Helicobacter antigens have been coated to a 96-well microtiter plate. The diluted dog serum/plasma sample is added to the wells of the coated plate.

• Qualitative
  The sample is added (diluted 1:30) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (> 1:90 > 1:270 > 1:810).

After incubation and washing, the bound dog antibodies are detected by a HRPO conjugated anti-species conjugate. The color reaction in the wells is directly related to the concentration of Helicobacter antibodies in the serum/plasma sample.

Protocol

Helicobacter ab