Intended use of the test kit
The feline Toxoplasma SRE test is designed to detect antibodies against toxoplasma infections. After washing the strips are incubated with the cat sera to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound cat antibodies to toxoplasma antigen. After incubation and rinsing the substrate is added and the optical density is measured at 620 nm.
Principle of the test kit
The test is based on the reaction of toxoplasma proteins with polyclonal cat antibodies. To this end toxoplasma proteins have been coated to a microtiter plate.
The diluted cat serum/plasma sample is added to the wells of the coated plate. After washing the bound cat antibodies are detected by a HRPO conjugated anti-species conjugate.
The colour reaction in the wells is directly related to the concentration of toxoplasma antibodies in serum/plasma samples.
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