Feline Panleukopenia Virus antigen ELISA

Description

Intended use of the test kit

To capture circulating FPV in fecal material the faeces is slightly diluted into buffer. To capture the FPV antigen, strips are coated with monoclonal antibodies which capture circulating antigen. After washing, HRPO labelled monoclonal antibody is added which will bind to the captured antigen. After washing and adding of substrate, a color reaction will show the bound antigen.

Principle of the test kit

The principle of the test is based on the reaction of two monoclonal antibodies with 2 different antigenic determinants of FPV. One monoclonal antibody, coated to the plate, catches the Panleucopenia Virus in the faeces sample after which the other, enzyme-labeled antibody detects the bound virus.

• Qualitative
  The sample is added (diluted 1:1) to the wells of the coated plate.

After washing, the bound feline antigen is detected by a HRPO conjugated anti-parvo conjugate.
The color reaction in the wells is directly related to the concentration of FPV antigen in the faeces sample.

Protocol

Feline Panleucopenia Virus antigen ELISA