Intended use of the test kit
The FeLV-p27 antigen SRE is designed to detect p27 antigen in individual serum/plasma samples. For this purpose monoclonal anti-FeLV antibodies attached to the plate will catch the viral-antigen in the sample to be tested. After incubation, the bound antigen is detected by use of a polyclonal HRPO anti-FeLV conjugate. After incubation and washing the substrate is added.
The colour development is directly correlated with the quantity of the p27 antigen in serum or plasma samples.
Principle of the test kit
The test is based on the reaction of FeLV-p27 antigen with monoclonal anti-FeLV-p27 antibodies. To this end these monoclonal antibodies are coated to a 32-well microtiter strip-plate.
The cat serum sample is added (diluted 1:1) to the wells of the coated plate. After incubation, the bound p27 antigen is detected by a polyclonal anti-FeLV-p27 HRPO conjugate.
Bound conjugate is made visible by adding substrate/chromagen mix. Intensity of the colour reaction in the wells is directly correlated to the concentration of p27 antigen in the serum sample.