Feline Immunodeficiency Virus IgG antibody ELISA

Description

Intended use of the test kit

The FIV p17/p24 ELISA is designed to detect antibodies against these proteins. To this end recombinant p17/p24 proteins are attached to the solid phase. After washing, the plates are incubated with the samples to be tested. The plates are washed after incubation to remove unbound materials. An anti-species conjugate is added to detect bound cat antibodies to FIV p17/p24. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of FIV proteins with cat antibodies. To this end, p17/p24 expression proteins have been coated to a 96 well microtiter strip plate.

• Qualitative
  The sample is added (diluted 1:100) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:100 (> 1:300 > 1:900 > 1:2700).

After washing, the bound cat antibodies are detected by an anti-species conjugate.
Bound anti-species conjugate is made visible by adding substrate/chromogen mix.
The intensity of the color reaction in the wells is directly correlated to the concentration of anti-FIV p17/p24 antibodies in the serum or plasma sample.

Protocol

Feline Immunodeficiency Virus antibody ELISA