Intended use of the test kit
The EVL SRE test detects antibodies against p24. To this end purified recombinant p24 proteins are attached to the solid phase. After washing, the wells are incubated with the cat sera to be tested. After washing HRPO conjugate is added to detect the bound antibodies.
Principle of the test kit
The test is based on the reaction of FIV proteins with cat antibodies. To this end, p24 expression proteins have been coated to a 32-well microtiter strip plate.
The cat serum sample is added (diluted 1:100) to the wells of the coated plate.
After washing, the bound cat antibodies are detected by an anti-species conjugate.
Bound anti-species conjugate is made visible by adding substrate/chromagen mix.
The intensity of the colour reaction in the wells is directly correlated to the concentration of anti-FIV p24 antibodies in the serum sample.