Encephalitozoon Cuniculi IgG antibody ELISA

Description

Intended use of the test kit

The E. cuniculi ELISA test kit is designed to detect antibodies against E. cuniculi proteins.
E. cuniculi proteins are attached to the solid phase. After washing the strips are incubated with the rabbit sera to be tested. The strips are washed after incubation to remove unbound materials. A HRPO conjugated anti-species antibodies conjugate is added to detect bound rabbit antibodies to E. cuniculi proteins. After incubation and rinsing the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of E. cuniculi proteins with rabbit antibodies.
To this end E. cuniculi proteins have been coated to a 96-well microtiter plate.

• Qualitative
  The sample is added (diluted 1:300) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution starting 1:100 (> 1:300 > 1:900 > 1:2700).

After washing the bound rabbit antibodies are detected by a HRPO conjugated anti-species antibodies conjugate.
The color reaction in the wells is directly related to the concentration of E. cuniculi antibodies in the serum/plasma sample.

Protocol

Encephalitozoon Cuniculi Antibody ELISA