Intended use of the test kit
This diagnostic test is intended to identify BLV-p24 antibodies in individual serum samples. This test which make use of monoclonal antibodies against P24 BLV antigen, this monoclonal antibody-mediated ELISA gives minimum non-specific reactions. This ELISA has a similar specificity to the agar gel precipitation test (AGPT), but is more sensitive.
Principle of the test kit
The test is based on the reaction of monoclonal antibodies to this end BLV P24 monoclonal antibodies have been coated to a 96-well microtiter strip plate. The serum is added to the wells together with BLV antigen to the monoclonal antibody coated plate.
The serum sample is added (diluted 1:1) to the wells of the coated plate.
The serum sample also can be titrated using a 3-step dilution, starting with undiluted (>1:3 >1:9 >1:27).
After incubation and washing, the bound monoclonal antibodies are detected by streptavidin HRPO conjugate.
Blocking of the color reaction in the wells is directly related to the presence of BLV p24 antibodies in the samples.