Intended use of the test kit
This is an direct ELISA with anti-species conjugate. The wells of the microtiter plate are coated with purified inactivated SVDV antigen. Diluted plasma/serum is added to the wells, after incubation for 1 hours at 37°C, the plate is washed. Conjugate is added for 1 hour at 37°C. After washing substrate is added. If the test serum is positive for SVDV antibodies, there will appear a colour reaction first blue and after adding stop solution, a yellow colour shall appear. This is an indication that the test is positive, the serum contains antibodies against SVDV. In case a serum sample is negative, no colour or a light yellow colour will appear, this indicated that there are no antibodies present reactive with SVDV.
Principle of the test kit
The principle of the test is based on the reaction of SVDV antigen bound to the solid phase and another monoclonal detecting antibody which detects pig antibodies bound to the viral antigen.
The sample is added (diluted 1:50) to the wells of the coated plate.
The sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (> 1:150 > 1:450 > 1:1350).
After incubation and washing, a monoclonal anti-pig IgG antibody conjugate is added.
After incubation and washing the bound conjugate can be detected by a color reaction.
Color reaction in the wells is directly related to the concentration of the SVDV antibodies in the serum or plasma sample.