Intended use of the test kit
The Salmonella ELISA test kit is based on purified antigens of Salmonella, which are coated to the solid phase. After the antigen/antibody reaction, the attached antibodies can be detected by use of a polyclonal conjugate.
Principle of the test kit
The Salmonella ELISA test kit is based on the reaction of Salmonella Polysaccharide with Salmonella antibodies. To this end purified Polysaccharide have been coated to a 96-well microtiter plate.
The dog sample is added (diluted 1:150) to the wells of the coated plate.
The dog sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (> 1:150 > 1:450 > 1:1350).
After washing, the bound antibodies are detected by a HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of antibodies in the serum or plasma.