Intended use of the test kit
The Salmonella Dublin antibody ELISA test kit is designed to detect antibodies against Salmonella Dublin. Purified Salmonella Dublin proteins are attached to the solid phase. After washing, the strips are incubated with the feces to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound antibodies. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.
Principle of the test kit
The principle of the test is based on the reaction of Salmonella Dublin proteins with polyclonal antibodies of IgA subclass in feces. To this end, Salmonella Dublin proteins have been coated to a 96 well micro titer plate.
The sample is added (diluted 1:6) to the wells of the coated plate.
The sample also can be titrated using a 3-step dilution, starting with a dilution 1:2 (> 1:6 > 1:18 > 1:54).
After washing, the bound antibodies are detected by a HRPO conjugated anti-species conjugate.
Color reaction in the wells is directly related to the concentration of Salmonella Dublin antibodies in the feces sample.