Rabies virus antibody ELISA

Description

Intended use of the test kit

This diagnostic test-system for the establishment of Rabies infection is intended to identify antibodies against neutralizing epitopes of rabies virus in serum or plasma samples. In contrast to other test systems this standardized ELISA based on synthetic peptides, has a very high sensitivity and specificity.

Principle of the test kit

The test is based on the reaction of synthetic peptides with polyclonal mouse antibodies.
To this end synthetic peptides have been coated to a 96-well microtiter strip plate. The mouse serum is added to the wells of the coated plate.

• Qualitative
  The sample is added (diluted 1:30) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (> 1:90 > 1:270 > 1:810).

After washing, the bound dog antibodies are detected by HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of rabies virus antibodies in the serum or plasma sample.

Protocol

Rabies Virus Antibody