Intended use of the test kit
This diagnostic test-system is intended to identify antibodies against epitopes of PVC2, in serum or plasma samples. In contrast to other test systems this standardized ELISA based on recombinant protein, and shows both very high sensitivity as well as very high specificity for PVC2.
Principle of the test kit
The test is based on the reaction of semi purified virus with polyclonal porcine antibodies.
To this end purified porcine antigen have been coated to a 96-well microtiter strip plate.
The sample is added (diluted 1:100) to the wells of the coated plate.
The sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (> 1:150 > 1:450 > 1:1350).
After washing, the bound porcine antibodies are detected by HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of porcine circovirus antibodies in the serum or plasma sample.