Canine Para-Influenza type 3/5 (PI3/5) IgG antibody ELISA

Description

Intended use of the test kit

The PI3/5 ELISA test kit is designed to detect antibodies against PI3/5 proteins (mostly glycoproteins). PI3/5 proteins are attached to the solid phase. After washing, the strips are incubated with the samples to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound dog antibodies to PI3/5 proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of PI3/5 proteins (mostly glycoproteins) with polyclonal dog antibodies. To this end PI3/5 proteins have been coated to a 96-well microtiter strip plate.

• Qualitative
  The sample is added (diluted 1:100) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (> 1:90 > 1:270 > 1:810).

After washing, the bound dog antibodies are detected by HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of PI3/5 antibodies in the sample.

Protocol

Para-Influenza Virus Antibody