Intended use of the test kit
This diagnostic test is intended to identify antibodies against sugar antigens of Pasteurella multocida, in serum samples. In contrast to test systems which make use of agglutinating bacterial antigen, this partial monoclonal based ELISA has a very high sensitivity and specificity (according to SAT, E.C. and Weybridge standards).
Principle of the test kit
This test is based on the reaction of Pasteurella proteins with dog polyclonal antibodies. To this end Pasteurella proteins have been coated to a 96-well plate.
The serum sample is added (diluted 1:100) to the wells of the coated plate.
The serum sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (> 1:150 > 1:450 > 1:1350).
After incubation and washing, a monoclonal anti-bovine IgG antibody conjugate is added.
After incubation and washing, the bound conjugate can be detected by a color reaction.
Color reaction in the wells is directly related to the concentration of the Pasteurella antibodies in the serum sample.