Intended use of the test kit
This diagnostic test is intended to identify antibodies against the antigen of BHV-1 virus, in serum and plasma samples.
Principle of the test kit
The test is based on the reaction of anti-IBR antibodies with BHV-1 antigen. To this end these BHV-1 antigens are coated to a 96-well microtiter strip-plate. The bovine serum or plasma sample is added to the wells of the coated plate.
The sample is added (diluted 1:2) to the wells of the coated plate.
After incubation, an anti-BHV-1 conjugate is added to compete for the BHV-1 antigen that is coated to the 96-well microtiter strip-plate. The biotinalated conjugate is detected by use of streptividine HRPO. Bound conjugate is made visible by adding substrate/chromogen mix.
Decrease of the color reaction in the wells is directly correlated to the concentration of anti-IBR antibodies in the serum or plasma sample.