Intended use of the test kit
This One-Step Test is intended to use as practical/routine screening test that can be done in a few minutes. This test kit is designed to detect FeLV p27 antigen by use of a Rapid Immunochromatic Assay.
Principle of the test kit
This FeLV One-Step Test is based on a chromatographic test strip, a monoclonal antibody and a polyclonal antibody which react with different epitopes of the FeLV P27 antigen. The monoclonal antibodies are conjugated to colloidal gold particles and the polyclonal antibody is immobilized on the strip in the test zone “T”. FeLV p27 antigen in a sample that is applied to the strip at the sample zone “S” will bind to the gold particles which then migrate to zone “T”. A color change in zone “T” indicates a positive test. FeLV antigen is also immobilized on the strip in the control zone “C”, which binds the gold conjugate to indicate that the test is working properly.