Feline Immunodeficiency Virus-p24/p17 Blocking IgG antibody ELISA

Description

Intended use of the test kit

The FIV-p24/p17 ELISA kit is designed to detect antibodies against these proteins. To this end recombinant p24/p17 products are attached to the solid phase. After washing, the plates are incubated with the samples to be tested. The plates are washed after incubation to remove unbound materials. A HRPO labeled anti-p24/p17 conjugate is added to detect bound cat antibodies. If these are present, this HRPO conjugate cannot bind to the plate. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of p17/p24 proteins with polyclonal cat antibodies. To this end, p17/p24 expression proteins have been coated to a 96 well microtiter strip plate.

• Qualitative
  The sample is added (diluted 1:2,5) to the wells of the coated plate.

After washing, the bound cat antibodies are detected by an HRPO anti-p24/p17 conjugate.
Bound HRPO anti-p24/p17 conjugate is made visible by adding substrate/chromagen mix. The intensity of the color reaction in the wells is directly related to the concentration of anti-FIV p17/p24 antibodies in the serum or plasma sample.

Protocol

Feline Immunodeficiency Virus antibody ELISA