Feline Corona Virus IgG antibody ELISA

Description

Intended use of the test kit

The FCoV ELISA test kit is designed to detect antibodies against FCoV proteins (mostly glycoproteins). FCoV proteins are attached to the solid phase. After washing, the strips are incubated with the sample, to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound cat antibodies to FCoV proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of FCoV proteins (mostly glycoprotein’s) with polyclonal cat antibodies. To this end purified FCoV proteins have been coated to a 96-well microtiter plate.

• Qualitative
  The sample is added (diluted 1:150) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (> 1:90 > 1:270 > 1:810).

After washing the bound cat antibodies are detected by a HRPO conjugated anti-species conjugate.
The colour reaction in the wells is directly related to the concentration of FCoV antibodies in the sample.

Protocol

Feline Corona Virus