Avian Influenza Virus antigen ELISA

Description

Intended use of the test kit

The principle of the test is based on the reaction of two monoclonal antibodies with 2 different antigenic determinants of AIV- NP type A. One monoclonal antibody, coated to the plate, catches the Influenza virus in the feces sample after which the other enzyme-labeled antibody detects the bound virus.

Principle of the test kit

The principle of the test is based on the reaction of a group specific monoclonal antibodies bound on the solid phase and another monoclonal detecting antibody which detects different conserved epitopes.
The test is based on the reaction of Influenza antigen with monoclonal anti-Influenza antibodies.
To this end these monoclonal antibodies are coated to a 96 well microtiter strip plate.

• Qualitative
  The sample is added (diluted 1:2) to the wells of the coated plate.

After incubation and washing, the bound Influenza antigen is detected by a monoclonal anti-Influenza antibody.
After incubation and washing, the bound conjugate can be detected by a color reaction.
Color reaction in the wells is directly related to the concentration of the Influenza antigen in the feces sample.

Protocol

Influenza Virus ag ELISA