Canine Parvo Virus IgG antibody ELISA

Description

Intended use of the test kit

The principle of the CPV test kit is based on the detection of antibodies against Parvo virus. The Parvo virus is attached to the solid phase by use of a monoclonal antibody. After the attachment of the antigen (Parvo virus) sera or plasma containing antibodies are able to react with the antigen. After the antigen/antibody reaction, the attached antibodies can be detected by use of a monoclonal HRPO conjugate.

Principle of the test kit

The test is based on the reaction of CPV proteins with dog antibodies. To this end CPV proteins have been coated to a 96-well microtiter plate by use of monoclonal antibodies.

• Qualitative
  The dog sample is added (diluted 1:250) to the wells of the coated plate.
• Quantitative
  The dog sample also can be titrated using a 3-step dilution, starting with a dilution 1:100  (> 1:300 > 1:900 > 1:2700).

After washing the bound dog antibodies are detected by a HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of CPV antibodies in the serum/plasma sample.

Protocol

Canine Parvo virus ab