Intended use of the test kit
This One- Step Test is intended to use as practical/routine screening test that can be done in a few minutes. This test kit is designed to detect Dirofliaria Immitis antigen by use of a Rapid Immunochromatic Assay.
Principle of the test kit
The Heartworm One-Step is based on a chromatographic principle in which a monoclonal antibody reacts with antigen produced by the adult worm. A monoclonal antibody is conjugated to colloidal gold particles and a monoclonal antibody is immobilized on the test strip in the test zone “T”. Antigen in serum/plasma sample that is applied to the test strip at the sample zone “S”, will bind to the colloidal gold particles which then migrate to zone ”T”. A colour change in zone “T” indicates a positive test. Colloidal gold particles are also immobilized on the test strip in the control zone “C”, to indicate that the test is working properly.