Intended use of the test kit
The Brucella canis ELISA test kit is designed to detect antibodies against Brucella canis proteins. Brucella canis proteins are attached to the solid phase. After washing, the strips are incubated with the canine sera to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound canine antibodies to Brucella canis proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.
Principle of the test kit
The test is based on the reaction of Brucella canis proteins with polyclonal dog antibodies. To this end Brucella canis proteins have been coated to a 96-well microtiter plate.
The dog serum sample is added (diluted 1:200) to the wells of the coated plate.
The dog serum sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (>1:90 >1:270 >1:810).
After washing, the bound dog antibodies are detected by a HRPO conjugated anti-species conjugate. The color reaction in the wells is directly related to the concentration of Brucella canis antibodies in the serum/plasma sample.