Intended use of the test kit
The Borrelia ELISA test kit is designed to detect antibodies against Borrelia proteins. Borrelia proteins are attached to the solid phase. After washing, the strips are incubated with the dog sample to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound dog antibodies to Borrelia proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.
Principle of the test kit
The test is based on the reaction of Borrelia proteins with polyclonal dog antibodies. To this end Borrelia proteins have been coated to a 96-well microtiter plate.
The dog sample is added (diluted 1:150) to the wells of the coated plate.
The dog sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (>1:150 >1:450 >1:1350).
After washing, the bound dog antibodies are detected by a HRPO conjugated anti-species conjugate. The color reaction in the wells is directly related to the concentration of Borrelia antibodies in the serum/plasma sample.