Intended use of the test kit
This diagnostic test system for the establishment of Bordetella infection is intended to identify antibodies against Bordetella antigens, in serum and plasma samples. In contrast to other test systems this standardized ELISA based on partial purified inactivated Bordetella antigen, has a very high sensitivity and specificity.
Principle of the test kit
The test is based on the reaction of Bordetella extracted and purified antigens with polyclonal dog antibodies. To this end Bordetella antigens have been coated to a 96-well microtiter strip plate.
The dog sample is added (diluted 1:150) to the wells of the coated plate.
The dog sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (>1:150 >1:450 >1:1350).
After washing, the bound dog antibodies are detected by HRPO conjugated anti-species antibodies conjugate. The color reaction in the wells is directly related to the concentration of Bordetella antibodies in the serum or plasma sample.