Intended use of the test kit
This diagnostic test system is intended to identify ALV-p27 antigen in serum or egg samples, and in individual or pooled samples.
Principle of the test kit
The test is based on the reaction of ALV-p27 antigen with monoclonal anti-ALV-p27 antibodies. To this end these monoclonal antibodies are coated to a 96-well microtiter strip-plate.
The egg or serum sample is added (diluted 1:1) to the wells of the coated plate.
The egg or serum sample also can be titrated using a 3-step dilution, starting with a dilution undiluted (>1:3 >1:9 >1:27).
After incubation, the bound p27 antigen is detected by a monoclonal anti-ALV-p27 conjugate. Bound conjugate is made visible by adding substrate/chromagen mix. Intensity of the color reaction in the wells is directly correlated to the concentration of p27 antigen in the egg or serum sample.