Avian Influenza Virus antigen ELISA

Description

Intended use of the test kit

The influenza virus type A ELISA is designed to detect these viral proteins. To this end monoclonal antibodies are attached to the solid phase. The plates are incubated with the sample to be tested. The plates are washed after incubation to remove unbound materials. A monoclonal antibody against influenza conjugated with biotin is added to detect bound influenza virus to the antibodies on the solid phase. The plates are washed after incubation to remove unbound materials. Enzyme conjugated streptavidin is added to detect bound biotin conjugate. After incubation and washing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of antibodies with NP proteins. To this end, monoclonal antibodies have been coated to a 96 well microtiter strip plate.
The sample is added to the wells of the coated plate.

• Qualitative
  The sample is added (diluted 1:4) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:2 (> 1:6 > 1:18 > 1:54).

After washing, the bound antigen is detected by conjugate.
Bound conjugate is made visible by adding substrate/chromagen mix.
The intensity of the color reaction in the wells is proportional to the concentration NP-A antigen in the sample.

Protocol

Influenza Virus ag ELISA