Feline Immunodeficiency Virus p24 antigen ELISA

Description

Intended use of the test kit

This diagnostic test is intended for the detection of p24 nucleoprotein in tissue culture samples. This standardized ELISA is based on affinity purified polyclonal antibodies directed to p24.

Principle of the test kit

The test is based on the reaction of FIV p24 proteins with affinity purified HRPO labelled polyclonal antibodies. To this end, FIV p24 proteins have been attached to the solid phase. The tissue culture samples (diluted/undiluted) are added to the wells of a pre-incubation plate. Immediately the same volume of HRPO labelled conjugate is added. After pre-incubation the antigen/conjugate mixture is transferred to the (washed) coated microtiter plate.
After washing, a substrate/chromagen mix is added to detect the bound conjugate.
The intensity of the color reaction in the wells is inversely correlated to the concentration of FIV p24 in the sample.

Protocol

Feline Immunodeficiency Virus antigen ELISA