Humaan Toxoplasma SRE

Description

Intended use of the test kit

The Toxoplasma SRE test is designed to detect antibodies against toxoplasma infections. After washing the strips are incubated with the sera to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound human antibodies to toxoplasma antibodies. After incubation and rinsing the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of toxoplasma proteins with polyclonal sheep/cattle antibodies. To this end toxoplasma proteins have been coated to a microtiter plate. The diluted serum/plasma sample are added to the wells of the coated plate. After washing the bound  antibodies are detected by a HRPO conjugated anti-species conjugate. The color reaction in the wells is directly related to the concentration of toxoplasma antibodies in the serum/plasma samples.

Protocol