Feline Panleukopenia Virus IgG antibody ELISA

Description

Intended use of the test kit

The principle of the FPV test kit is based on the detection of antibodies against Panleukopenia virus. The Panleukopenia virus is attached to the solid phase by use of a monoclonal antibody. After the attachment of the antigen (Panleukopenia virus virus) sera or plasma containing antibodies are able to react with the antigen. After the antigen/antibody reaction, the attached antibodies can be detected by use of a monoclonal HRPO conjugate.

Principle of the test kit

The test is based on the reaction of FPV proteins with dog antibodies. To this end FPV proteins have been coated to a 96-wellmicrotiter plate by use of monoclonal antibodies.

• Qualitative
  The cat sample is added (diluted 1:250) to the wells of the coated plate.
• Quantitative
  The cat sample also can be titrated using a 3-step dilution, starting with a dilution 1:100 (> 1:300 > 1:900 > 1:2700).

After washing the bound cat antibodies are detected by a HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of FPV antibodies in the serum/plasma sample.

Protocol

Feline Panleukopenia virus ab