Feline Herpes Virus IgG antibody ELISA

Description

Intended use of the test kit

The FHV ELISA test is designed to detect antibodies against FHV proteins. FHV antigens (proteins/glycoproteins) are attached to the solid phase. After washing, the strips are incubated with the samples to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound cat antibodies to FHV proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of FHV proteins with polyclonal cat antibodies. To this end FHV antigens have been coated to a microtiter plate.
The diluted cat serum/plasma sample is added to the wells of the coated plate.

• Qualitative
  The sample is added (diluted 1:150) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (> 1:150 > 1:450 > 1:1350).

After washing the bound cat antibodies are detected by a HRPO conjugated anti-species conjugate
The color reaction in the wells is directly related to the concentration of FHV antibodies in serum/plasma samples.

Protocol

FHV