Canine Leptospira IgG antibody ELISA

Description

Intended use of the test kit

This diagnostic test for leptospirosis is intended to identify antibodies against sugar antigens of leptospira in serum and plasma samples. In contrast to test systems which make use of unpurified non-specific leptospira antigens, this test uses a monoclonal antibody which catches a specific leptospira sugar antigen. This monoclonal based ELISA has very high specificity and sensitivity.

Principle of the test kit

An antigen solution antibody mixture is coated to the wells of the micro titer plate after stabilization and drying plates are vacuum sealed. Diluted serum or plasma samples are added to the coated wells.

• Qualitative
  The sample is added (diluted 1:150) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (> 1:150 > 1:450 > 1:1350).

After incubation, a monoclonal anti-dog conjugate is added and the plates are again incubated. After incubation, substrate is added. Within several minutes the color reaction is stopped and the plates are immediately read at 450 nm.
Color reaction in the wells is directly related to the concentration of the Leptospira antibody in the sample.

Protocol

Leptospira antibody