Canine Corona Virus IgM antibody ELISA

Description

Intended use of the test kit

The CCV ELISA test kit is designed to detect antibodies against CCV proteins (mostly glycoproteins). CCV proteins are attached to the solid phase. After washing, the strips are incubated with the dog sample to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound dog antibodies to CCV proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of CCV proteins (mostly glycoproteins) with polyclonal dog antibodies. To this end CCV proteins have been coated to a 96-well microtiter plate.

Qualitative
The dog sample is added (diluted 1:60) to the wells of the coated plate.
Quantitative
The dog sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (>1:90 >1:270 >1:810).

After washing the bound dog antibodies are detected by a HRPO conjugated anti-species conjugate. The color reaction in the wells is directly related to the concentration of CCV antibodies in the serum/plasma sample.

Protocol

Canine Corona virus ab