Canine Corona Virus IgG antibody ELISA

Description

Intended use of the test kit

The CCV ELISA test kit is designed to detect antibodies against CCV proteins (mostly glycoproteins). CCV proteins are attached to the solid phase. After washing, the strips are incubated with the canine sample to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound canine antibodies to CCV proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of CCV proteins (mostly glycoproteins) with polyclonal dog antibodies. To this end CCV proteins have been coated to a 96-well microtiter plate.

• Qualitative
  The canine sample is added (diluted 1:150) to the wells of the coated plate.
• Quantitative
  The canine sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (>1:90 >1:270 >1:810).

After washing the bound canine antibodies are detected by a HRPO conjugated anti-species conjugate. The color reaction in the wells is directly related to the concentration of CCV antibodies in the serum/plasma sample.

Protocol

Canine Corona virus ab