Avian Leukaemia Virus antigen ELISA

Description

Intended use of the test kit

This diagnostic test system is intended to identify ALV-p27 antigen in serum or egg samples, and in individual or pooled samples.

Principle of the test kit

The test is based on the reaction of ALV-p27 antigen with monoclonal anti-ALV-p27 antibodies. To this end these monoclonal antibodies are coated to a 96-well microtiter strip-plate.

• Qualitative
  The egg or serum sample is added (diluted 1:1) to the wells of the coated plate.
• Quantitative
  The egg or serum sample also can be titrated using a 3-step dilution, starting with a dilution undiluted (>1:3 >1:9 >1:27).

After incubation, the bound p27 antigen is detected by a monoclonal anti-ALV-p27 conjugate. Bound conjugate is made visible by adding substrate/chromagen mix. Intensity of the color reaction in the wells is directly correlated to the concentration of p27 antigen in the egg or serum sample.

Protocols

Avian Leukaemia Virus ag HRPO RTU

Avian Leukaemia Virus ag HRPO RTU 5plate