Anti-Nuclear antibody ELISA

Description

Intended use of the test kit

The ANA ELISA test kit is designed to detect antibodies against ANA proteins (lysozyme, dsDNA etc). Purified ANA proteins are attached to the solid phase. After washing, the strips are incubated with the canine sera or plasma to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound canine antibodies to ANA proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of ANA proteins (mostly glycoproteins) with polyclonal dog antibodies. To this end ANA proteins have been coated to a 96-well microtiter plate.

Protocols

Anti Nuclear antibodies