Canine Distemper Virus IgM antibody ELISA

Description

Intended use of the test kit

The CDV test kit is based on monoclonal antibodies against a common epitopes of CDV, which are coated to the solid phase. The distemper virus is attached to the solid phase by the monoclonal antibody. After the attachment of the antigen (distemper virus), sera containing antibodies are able to react with the attached antigen. After the antigen/antibody reaction, the attached antibodies can be detected by use of a polyclonal conjugate.

Principle of the test kit

The test is based on the reaction of CDV proteins with CDV antibodies. To this end purified CDV proteins have been coated to a 96-well microtiter plate.

• Qualitative
  The dog sample is added (diluted 1:30) to the wells of the coated plate.
• Quantitative
  The dog sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (>1:90 >1:270 >1:810).

After washing the bound CDV antibodies are detected by a HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of CDV antibodies in the serum or plasma samples.

Protocol

Canine Distemper virus ab (IgM)