Bovine Leukemia P24/gp51 IgG antibody ELISA for serum/plasma samples

Description

Intended use of the test kit

This diagnostic test system for BLV infected cattle is intended to identify BLV p24 and gp51 antibodies in individual or pooled serum samples. In contrast to test systems which make use of polyclonal antibodies or only one gp51 monoclonal antibody, this two monoclonal antibodies mediated ELISA gives a minimum of non-specific reactions.

Principle of the test kit

The test is based on the reaction of anti-BLV-p24 and anti-BLV-gp51 antibodies in test samples with BLV antigen which have been coated to a 96 well microtiter plate.

• Qualitative
  The sample is added (diluted 1:30) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (>1:90 >1:270 >1:810).

After washing, test serum samples are added to the wells and if present anti-BLV-p24 and anti-BLV-gp51 antibodies from the samples will bind to the antigen. The complex is detected by a horseradish peroxidase (HRPO) conjugated monoclonal antibody directed against bovine IgG. Color reaction in the wells is directly related to the presence of BLV-p24 and BLV-gp51 antibodies in the sample.

Protocols

Bovine Leukaemia Virus p24-gp51

Bovine Leukaemia Virus p24-gp51 5- plate