Feline Calici Virus IgG antibody ELISA

Description

Intended use of the test kit

The FCV ELISA test is designed to detect antibodies against Feline Calici Virus proteins. To this end Feline Calici antigens (proteins/glycoproteins) are attached to the solid phase. The strips are washed after incubation to remove unbound materials. The samples to be tested are diluted and added to the wells and incubated. After incubation the strips are washed, a HRPO labelled anti-species conjugate is added to detect bound cat antibodies to FCV. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of FCV proteins with polyclonal cat antibodies. To this end FCV antigens have been attached to the solid phase.

• Qualitative
  The sample is added (diluted 1:150) to the wells of the coated plate.
• Quantitative
  The sample also can be titrated using a 3-step dilution, starting with a dilution 1:50 (1:150 > 1:450 > 1:1350).

After washing, the bound cat antibodies are detected by a HRPO conjugated anti-species conjugate The colour reaction is directly related to the concentration of FCV antibodies in serum/plasma samples.

Protocol

Feline Calici Virus